Real-time PCR results showing titre in copies/mg from tissue or flea samples of five red and four grey squirrels. Squirrel tissues tested were as follows: A (antebrachial skin), B (blood), C (cheek), IT (intestinal tract), Li (lip), LS (lip scab or ulcer), LN (submandibular lymph node), Lu (lung), N (nasal skin), SU (eyelid skin ulcer), S (facial skin) and additionally fleas (F) when present. Tissues are without gross lesions unless indicated. Positive samples are indicated in the figure, and are expressed as the average of three reactions. Those samples still testing equivocal on repeat are indicated by brackets. Samples testing negative are shown below the figure.