Skip to main content
Figure 2 | BMC Veterinary Research

Figure 2

From: Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrels

Figure 2

Melt curve analysis (derivative - rate of change of fluorescence against temperature) for positive and negative amplicons from the real-time assay. Amplicons from squirrel poxvirus gave a specific melt at approximately 88°C. Negative control samples gave distinguishably lower melt curves and are seen below between 76°C and 85°C.

Back to article page

BMC Veterinary Research

ISSN: 1746-6148

Contact us