Figure 2From: Detection of squirrel poxvirus by nested and real-time PCR from red (Sciurus vulgaris) and grey (Sciurus carolinensis) squirrelsMelt curve analysis (derivative - rate of change of fluorescence against temperature) for positive and negative amplicons from the real-time assay. Amplicons from squirrel poxvirus gave a specific melt at approximately 88°C. Negative control samples gave distinguishably lower melt curves and are seen below between 76°C and 85°C.Back to article page