Western blots comparing C-, L- and H-types. Western blots comparing reference samples of L-, C- and experimental H-type isolates (indicated by L, C and H). From top to bottom, the three bands of each sample correspond to di-, mono- and unglycosylated forms of the PrP. The different reaction of C-type in comparison to H-type is visible with 12B2 antibody (a), the higher molecular mass of H-type is revealed by Sha31 antibody (b) and the presence of a fourth band in H-type is shown by SAF84 antibody (c, arrow). L-type shows a greater proportion of monoglycosylated band compared to the diglycosylated band with Sha31 antibody than C-type. A molecular marker kit is displayed (MM, three arrow heads represent position of Mr 20, 30 and 40 kDa). The antibody concentrations used were 2 μg/ml for 12B2 and SAF84. For Sha31, the manufacturer's instructions were followed. The applied tissue equivalents are 7.1 mg of fresh brain per lane. The exposure time in the imager was 5 min.