Figure 2

Discriminatory Western Immunoblot for case 30842. Brain tissue homogenate of sheep 30842 along with BSE-positive, scrapie-positive and negative controls was digested with proteinase K and MAbs P4 and 6H4 were used for PrP^res detection and discrimination of BSE and scrapie by Western immunoblot. The pattern of the di-, mono- and non- glycosylated moieties of case 30842 shows typical features of classical scrapie with regard to molecular mass of non-glycoslated PrP^res (lower band) and the reactivity with the n-terminal MAb P4.