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Table 2 Results of rapid test (ELISA TeSeE), immunoblot (TeSeE Western Blot), IHC (cocktail of 2G11 and F89/160.1.5) and PrP genotypes of AS cases.

From: Atypical/Nor98 scrapie in the Basque Country: a case report of eight outbreaks

  2004 2005 2008 2009
  M7 M72 M45 M31 M15 M19-1 M27 M9-2
Origin Fallen stock Fallen stock Slaughtered Slaughtered Fallen stock (Advanced autolysis) Fallen stock Slaughtered Slaughtered
Month of death June November April January February December February April
Province Vizcaya Alava Guipuzcoa Guipuzcoa Alava Guipuzcoa Guipuzcoa Guipuzcoa
Age (years) 6.3 12.8 4.3 7.0 9.1 5.6 8.1 10.3
Genotype AFRQ ALRQ ALRR ALRQ AFRQ ALRQ AFRQ AFRQ AFRQ AFRQ AFRQ ALHQ ALRQ ALRH ALRQ ALRQ
Clinical symptoms Not registered Not registered Not registered Yes Not registered Not registered Not registered Not registered
Rapid test result (cut off) 0.377 (0.218) 0.387 (0.219) 1.112 (0.219) 2.788 (0.230) 2.859 (0.230) 2.710 (0.219) 0.498 (0.102) 1.289 (0.103)
WB TeSeE         
Obex-Cerebellum 1 2 3 2 3 1 1 2
Cerebellum 0 0 3 3 1 3 1 3
Cerebral Cortex 0 0 1 0 2 1 1(3)* 2
IHC         
MO PC NA NA 2 1 1 1 1
Cerebellum 1 3 3 3 1 3 1 3
Frontal Lobe PC NA 2 NA 3 1 2 1
  1. Numbers on WB column indicates the signal intensity according to Figure 1. 0: negative, 1: faint, 2: moderate and 3: intense. NA: Not available. PC: Poor condition. MO: Medulla oblongata. Immunohistochemical staining was graded subjectively for intensity and spread from 0 to 3 as in WB. * Refers to PrPSc signal after concentrating the sample 10 folds.