Figure 2

Mammary gland, dog. Immunohistochemical detection of HEPACAM1 protein. (A) Non neoplastic mammary gland with membrane-bound expression of HEPACAM1 (right) and adenoma with complete loss of HEPACAM1 expression (left). Bar = 1250 μm. (B) In contrast, almost all carcinomas expressed membrane-bound HEPACAM1. Bar = 250 μm (C) Intravascular tumour cells expressed HEPACAM1 at their cell membrane. Bar = 200 μm (D) The majority of tumour cells lymph node metastases of mammary carcinomas expressed membrane bound HEPACAM1 with increased expression at sites of cell to cell contact. Bar = 300 μm Haematoxylin counterstain.