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Table 3 Primers used in this study for MLVA and SNR analyses

From: SNR analysis: molecular investigation of an anthrax epidemic

 

LOCUS

PRIMER SEQUENCE (5' to 3')

CONCENTRATION μM

MLVA 25

   
 

CG3

F:CY5.5-TGTCGTTTTACTTCTCTCTCCAATAC

R:AGTCATTGTTCTGTATAAAGGGCAT

0.30

 

bams44

F: CY5.5-GCACTTGAATATTTGGCGGTAT

R: GCGAATTAATTGCTCCTCAAAT

0.30

 

bams3

F: CY5.5-GCAGCAACAGAAAACTTCTCTCCAATAACA

R:TCCTCCCTGAGAACTGCTATCACCTTTAAC

0.30

Multiplex A

vrrB2

F: D2-CACAGGCTATTCTTTATCAAACTCATC

R: CCCAAGGTGAAGATTGTTGTTGA

0.15

 

bams5

F: D2-GCAGGAAGAACAAAAGAAACTAGAAGAGCA

R: ATTATTAGCAGGGGCCTCTCCTGCATTACC

0.30

 

bams15

F: D2-GTATTTCCCCCAGATACAGTAATCC

R: GTGTACATGTTGATTCATGCTGTTT

0.60

 

bams1

F: CY5-GTTGAGCATGAGAGGTACCTTGTCCTTTTT

R: AGTTCAAGCGCCAGAAGGTTATGAGTTATC

0.15

 

vrrC1

F: CY5-GAAGCAAGAAAGTGATGTAGTGGAC

R: CATTTCCTCAAGTGCTACAGGTTC

0.30

 

bams13

F: CY5.5-AATTGAGAAATTGCTGTACCAAACT

R: CTAGTGCATTTGACCCTAATCTTGT

0.30

 

vrrB1

F: CY5--ATAGGTGGTTTTCCGCAAGTT

R: GATGAGTTTGATAAAGAATAGCCTGTG

0.10

Multiplex B

bams28

F: CY5-CTCTGTTGTAACAAAATTTCCGTCT

R: TATTAAACCAGGCGTTACTTACAGC

0.15

 

vrrC2

F: CY5-CCAGAAGAAGTGGAACCTGTAGCAC

R: GTCTTTCCATTAATCGCGCTCTATC

0.10

 

bams53

F: D2-GAGGTGTGTTAGGTGGGCTTAC

R: CATATTTTCACCTTAATTTTGGAAG

0.60

 

bams31

F: D2-GCTGTATTTATCGAGCTTCAAAATCT

R: GGAGTACTGTTTGTTGAATGTTGTTT

0.60

 

vrrA

F: CY5.5-CACAACTACCACCGATGGCACA

R: GCGCGTTTCGTTTGATTCATAC

0.06

 

bams25

F: CY5.5-CCGAATACGTAAGAAATAAATCCAC

R: TGAAAGATCTTGAAAAACAAGCATT

0.15

Multiplex C

bams21

F: CY5.5-TGTAGTGCCAGATTTGTCTTCTGTA

R: CAAATTTTGAGATGGGAGTTTTACT

0.30

 

bams34

F: D2-TGTGCTAAATCATCTTGCTTGG

R: CAGCAAAATCAATCGAATCAAA

0.30

 

bams24

F: CY5-CTTCTACTTCCGTACTTGAAATTGG

R: CGTCACGTACCATTTAATGTTGTTA

0.30

 

bams51

F: CY5-ATTTCCTGAAGCAGGTTGTGTT

R: TGCATCTAACAATGCAGAACAA

0.60

 

bams22

F: CY5-ATCAAAAATTCTTGGCAGACTGA

R: ACCGTTAATTCACGTTTAGCAGA

0.15

Multiplex D

bams23

F: D2-CGGTCTGTCTCTATTATTCAGTGGT

R: CCTGTTGCTCCTAGTGATTTCTTAC

0.30

 

bams30

F: CY5.5-AGCTAATCACCTACAACACCTGGTA

R: CAGAAAATATTGGACCTACCTTCC

0.30

 

pXO1

F: CY5-CAATTTATTAACGATCAGATTAAGTTCA

R: TCTAGAATTAGTTGCTTCATAATGGC

0.15

 

pXO2

F: CY5.5-TCATCCTCTTTTAAGTCTTGGGT

R: GTGTGATGAACTCCGACGACA

0.15

STR-MTD

   

Singleplex A

CL33

F: 6FAM-TGGGGTATATTCCCATCGAA

R:CCGCAGATACCAACCAACAT

0.2

Singleplex B

CL12

F: 6FAM-AAGCCAGGTGCAAAAACAGT

R: TCTCACTGTGCCTCGCTAAA

0.2

Singleplex C

CL1

F: 6FAM-TTCTCGGAGATGATTTTCGG

R:CTCCCATTTTACATCCCCCT

0.2

Singleplex D

CL37

F: NED-CTCCGCAATTTTCAAACGAT

R: CCGCCGGCATAAAGATAGTA

0.2

KEN-MTD

   
 

CL33

HM1

F: PET GAAAACTTTGCAACCGACC

R: GTCGAACGTGTTCTAGCTACAG

0.2

Multiplex I

CL10

HM6

F: 6FAM-TAAAAAGACAGAATTTTCAATTTTATCAACAAC

R: GTGGAAACTAATGTGAGTTATATATGTTAGTTAAG

0.2

 

CL12

HM2

F: VIC-GCTATTCTCACTGTGCCTCG

R: GTTAAAACGAAGTAAAGAAAAGTGGG

0.2

 

CL35

HM13

F: NED-GGATTGCTTAAGGTATATAATGGATTT

R: GTTGTGTTCCATATGTATCCCTCC

0.1