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Table 4 Real time quantitative RT-PCR Results of the top ten up- and down-regulated genes measured by the microarray analysis.

From: Gene expression profiling in equine polysaccharide storage myopathy revealed inflammation, glycogenesis inhibition, hypoxia and mitochondrial dysfunctions

Gene

Normalized ratio of microarray analysis

Relative expression ratio of quantitative RT-PCR

RT-PCR ratio different from 1 p-value

UP

   

IL18

6.20

4.29

0.01

CTSS

3.17

1.61

0.05

LUM

3.15

1.75

0.05

CD44

2.42

2.87

0.01

FN1

2.17

0.72

0.05

GSTO1

2.03

3.23

0.01

VCAM1

1.98

7.21

0.01

ITGB1

1.87

0.09

0.05

CXCR4

1.81

3.43

0.01

HIF1A

1.73

2.99

0.01

DOWN

   

NFKBIB

0.59

0.60

0.05

PLCG2

0.58

0.97

NS

ND3

0.57

0.60

0.05

SLC25a17

0.57

1.77

0.05

SRI

0.57

0.98

NS

ND5

0.54

0.49

0.05

PDE4D

0.54

0.67

0.05

SLC25a15

0.48

0.96

NS

VEGF

0.37

0.35

0.05

PRKCA

0.37

0.16

0.05

SLC2A2

0.35

0.79

0.05

  1. One sample T-test compared the relative expression ratio of RT-PCR results against 1. For p > 0.05,
  2. P-values were non-significant (NS).