Gene expression profiling in equine polysaccharide storage myopathy revealed inflammation, glycogenesis inhibition, hypoxia and mitochondrial dysfunctions

Barrey, Eric; Mucher, Elodie; Jeansoule, Nicolas; Larcher, Thibaut; Guigand, Lydie; Herszberg, Bérénice; Chaffaux, Stéphane; Guérin, Gérard; Mata, Xavier; Benech, Philippe; Canale, Marielle; Alibert, Olivier; Maltere, Péguy; Gidrol, Xavier

doi:10.1186/1746-6148-5-29

Table 1 List of the primers used for real time quantitative RT-PCR.

From: Gene expression profiling in equine polysaccharide storage myopathy revealed inflammation, glycogenesis inhibition, hypoxia and mitochondrial dysfunctions

Gene	Primer name	Primer sequence	Length	Salt Tm	GC%
IL18	IL18_1Fwd	TGCATTAGCTTGGTGGAAATG	21	61	42
	IL18_1Rev	AGGTTCAAGCCTGCCAAAGT	20	61	50
CTSS	CTSS_1Fwd	ACAACGGCATCGATTCAGAC	20	61	50
	CTSS_1Rev	GCCAAAGGGAAGTTCGGTAT	20	60	50
LUM	LUM_1Fwd	CCTGGAGGTCAATGAACTTGAA	22	61	45
	LUM_1Rev	ACGCAAATGCTTGATCTTGG	20	61	45
CD44	CD44_1Fwd	GATCCACCCCAACTCCATCT	20	61	55
	CD44_1Rev	TGACCGAGGTGCAGTCTTCT	20	61	55
FN1	FN1_1Fwd	CCCTGGTGTCACAGAGGCTA	20	61	60
	FN1_1Rev	AGTTGGGGAGGCTCATCTGT	20	61	55
GSTO1	GSTO1_1Fwd	TACCTCATCTGGCCCTGGTT	20	62	55
	GSTO1_1Rev	ATGGCTTCCATCCAGAGCTT	20	61	55
VCAM1	VCAM1_1Fwd	GCACTGCCATTGAGATGTGA	20	61	50
	VCAM1_1Rev	TGCAATGTTGCTATGGCTCA	20	61	45
ITGB1	ITGB1_1Fwd	TCTCCAGAAGGTGGCTTTGA	20	61	50
	ITGB1_1Rev	TCCGTGGAAAATACCAGCAG	20	61	50
CXCR4	CXCR4_1Fwd	GCAGCAGCAGGTAGCAAAGT	20	61	55
	CXCR4_1Rev	CCACGTCATCCTCCGTGTAG	20	61	60
HIF1A	HIF1A_1Fwd	GGACTCGGATCATCTGACCA	20	61	55
	HIF1A_1Rev	TATCCCCCTCTTTTGGCAAG	20	61	50
NFKBIB	NFKBIB_1Fwd	CGTCATCCACAAAGATGCAG	20	60	50
	NFKBIB_1Rev	CTGGGCCTCAACAGCCTAGT	20	62	60
PLCG2	PLCG2_1Fwd	CATCTTGTACGGCACCCAGT	20	61	55
	PLCG2_1Rev	TTCAAGCCAGACAGCCACTT	20	61	50
ND3	ND3fwd	CCACAACTAAACATCTATGCAGAAAAA	27	62	33
	ND3rev	GGAGGCGTGCTGACCCTAT	19	62	63
SLC25a17	SLC25a17_1Fwd	GCAATCTGGGTCAAAGGTCA	20	61	50
	SLC25a17_1Rev	GCCCCTTGCAGCTTTAGTCT	20	61	55
SRI	SRI_1Fwd	ATTGCCGGAGGATACAAACC	20	61	50
	SRI_1Rev	GCCATTCAGTACAGCCCAGA	20	61	55
ND5	ND5fwd	GATGATGATACGGCCGAACAG	21	61	52
	ND5rev	CCCGATGCGGTTATAAAGGA	20	58	50
PDE4D	PDE4D_1Fwd	AGCCTGCGAACTGTACGAAA	20	61	50
	PDE4D_1Rev	ATGGATGGTTGGTTGCACAT	20	61	45
SLC25a15	SLC25a15_1Fwd	TGAACTGAGCCGATCGTTTT	20	61	45
	SLC25a15_1Rev	CAGAGGCAGATTCCACCAAC	20	61	55
VEGF	VEGF_1Fwd	TCTACCTCCACCATGCCAAG	20	61	55
	VEGF_1Rev	GTCTCGATTGGACGGCAGTA	20	61	55
PRKCA	PRKCA_1Fwd	GACTTCATGGGCTCCCTTTC	20	61	55
	PRKCA_1Rev	TCTTCGTCCCCTTCTGGAAT	20	61	50
SLC2A2	SLC2A2_1Fwd	GCCATCGGTACTCTTCACCA	20	61	55
	SLC2A2_1Rev	TGTGCCACAGCTTCTGATTG	20	61	50

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ISSN: 1746-6148

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