Figure 1
From: Experimental infection of Foxes with European bat Lyssavirusestype-1 and 2
Kinetics of EBLV-1 and CVS-11 neutralizing activity for foxes intramuscularly infected with EBLV-1 and EBLV-2. Neutralising activity is expressed by log(ED50): dilution giving a 50% neutralization of rabies virus (CVS-11/EBLV-1). Figure 1A: mean of VNA response of 5 foxes intramuscularly inoculated with 103.5 MIC LD50 of EBLV-2 (group 1). The 5 negative controls did not develop any rabies neutralising activity during the entire experiment (log(ED50) < 0.24, data not shown). Figure 1B: mean of VNA response obtained in the second trial with intramuscular inoculation of different doses of EBLV-1 (group 3). One fox received 104,7 MIC LD50, one 104,4 and 2 groups of 2 received respectively 103.7 and 102.7. One negative control did not develop any rabies neutralising activity all over the experiment (log (ED50)< 0.24, data not shown). Figure 1C: VNA response obtained from the fourth IM trial with EBLV-1 (group 4). 3 groups of 5 animals received respectively the final dose of 4.4, 3.7 and 3 logs MIC LD50. One negative control fox did not develop rabies neutralising activity all over the entire experiment (log(ED50) < 0.24, data not shown). Remark: All neutralising activities are expressed with log(ED50) due to the absence of an available specific reference serum of known neutralizing titre against EBLVs.