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Table 3 Primer sequences and probes for real-time PCR assays.

From: Epithelial expression of mRNA and protein for IL-6, IL-10 and TNF-α in endobronchial biopsies in horses with recurrent airway obstruction

Gene

Forward primer 5' to 3'

Reverse primer 5' to 3'

Probe 5' to 3'

GAPDH a

AAG TGG ATA TTG TCG CCA TCA AT

AAC TTG CCA TGG GTG GAA TC

TGA CCT CAA CTA CAT GGT CTA CAT GTT TCA

IL-6

AGC AAG TGT GAA AAC AGC AAG

CAT CAG GCA GGT CTC CTG AT

CTG GCA GAA AAC AAC CTG AAT CTT CCA

IL-10

TTC AGC AGG GTG AAG ACT TTC

CTT GGC AAC CCA GGT AAC CCT TA

TGT TGA ACG GGT CCC TGC TGG AG

TNF-α

GCT CCA GAC GGT GCT TGT G

GCC GAT CAC CCC AA AG TG

TGT CGC AGG AGC CAC CAC GCT

  1. Primers and Probes designed from nucleotide sequence for this study. aD.M. Ainsworth et al. Recurrent airway obstruction (RAO) in horses in characterised by IFN-γ and IL-8 production in bronchoalveolar lavage cells. In: Veterinary Immunology and Immunopathology 96 (2003) 83–91.
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BMC Veterinary Research

ISSN: 1746-6148

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