Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil

Table 2 Reverse transcription polymerase chain reaction results from 3 reference strains and 2 previously characterized field isolates.

Sample no.	Description	Serotype	Ct values*
P7	Reference strain – O1 Campos	O	14.53
P8	Reference strain – A24 Cruzeiro	A	13.17
P9	Reference strain – C3 Indaial	C	34.92
P10	Field isolate (Mato Grosso do Sul, 2005)	O	17.12
P11	Field isolate (Roraima, 1999)	A	18.50

* Ct (cycle threshold) corresponds to the number of cycles required for a given sample to reach the threshold above which it is considered positive. A sample was considered positive when the Ct value was lower than 40.

ISSN: 1746-6148