Skip to main content

Table 1 Positive and inconclusive results by real-time polymerase chain reaction and the corresponding virus isolation and enzyme-linked immunosorbent assay (ELISA) results.*

From: Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil

   Virus isolation
Sample no. Ct value CPE ELISA
527 42.22 Negative ND
567 44.26 Negative ND
605 44.26 Positive Negative
613 34.94 Positive Negative
652 41.52 Negative ND
674 36.60 Positive Positive (type O)
675 35.20 Positive Positive (type O)
  1. * Origin of samples was Eldorado/Japorã, State of Minas Gerais. Ct (cycle threshold) corresponds to the number of cycles required for a given sample to reach the threshold above which it is considered positive. A sample was considered positive when the Ct value was lower than 40, and inconclusive when the Ct value was higher than 40. CPE = cytopathic effect; ND = not determined. Only samples that resulted in CPE were processed for indirect sandwich ELISA.