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Table 1 Average OD readings for serum dilution and protein G combination.

From: Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovisin european wild boar

   Serum dilution Protein G dilution
   1/10 1/100 1/200 1/500 1/1000 1/2500 2.5 μg/ml 1 μg/ml 0.5 μg/ml 0.05 μg/ml
WB1 + WB4 S2/S1 9,2805 9,2610 9,6143 9,5895 9,1172 6,4596 6,3067 8,3368 10,702 11,129
  S3/S1 10,465 11,061 11,38 11,574 11,730 9,1666 7,5116 10,252 12,074 13,751
  S1 0,1758 0,1089 0,0855 0,0596 0,0465 0,0355 0,1428 0,0981 0,0728 0,0275
  S2 1,0053 0,8198 0,7176 0,57 0,4293 0,2521 0,7832 0,729 0,6658 0,3514
  S3 1,0706 0,9049 0,7892 0,6605 0,5357 0,3438 0,8763 0,8273 0,7576 0,4086
WB2 S2/S1 1,4873 1,2624 1,2840 1,1513 1,2033 1,1837 1,2756 1,2966 1,3386 1,1372
  S3/S1 5,9384 5,5608 5,0313 3,6979 2,8577 2,3318 4,0252 4,7892 5,1064 3,0244
  S1 0,0941 0,0669 0,055 0,0523 0,0414 0,0305 0,094 0,062 0,0491 0,0217
  S2 0,1473 0,0841 0,0719 0,061 0,0504 0,0353 0,125 0,0844 0,0657 0,0248
  S3 0,5771 0,3818 0,2885 0,2023 0,1224 0,0686 0,4152 0,3324 0,2779 0,0683
WB2 S2/S1 3,1229 2,4333 2,6135 2,0944 1,8366 1,4173 2,2195 2,3216 2,5731 1,8978
  S3/S1 4,0662 3,3376 4,0625 3,1850 2,8014 2,1894 3,0310 3,3525 3,7237 2,9875
  S1 0,1119 0,0671 0,054 0,0611 0,0485 0,0383 0,1052 0,0704 0,0543 0,0241
  S2 0,3505 0,1645 0,145 0,1318 0,0914 0,0543 0,2504 0,8477 0,1515 0,0464
  S3 0,41 0,2215 0,2269 0,1994 0,1374 0,0839 0,3213 0,8577 0,213 0,0731
  1. All protein G concentrations at each serum dilution, and all serum dilutions at each protein G concentration.
  2. WB1+WB4: Sum of wild boar 1 and wild boar 4 results. S2/S1 and S3/S1: Increase rate between pre- and post-immunization results. S1, S2 and S3: OD values.