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Table 1 Average OD readings for serum dilution and protein G combination.

From: Development and validation of an enzyme-linked immunosorbent assay for antibodies against Mycobacterium bovisin european wild boar

  

Serum dilution

Protein G dilution

  

1/10

1/100

1/200

1/500

1/1000

1/2500

2.5 μg/ml

1 μg/ml

0.5 μg/ml

0.05 μg/ml

WB1 + WB4

S2/S1

9,2805

9,2610

9,6143

9,5895

9,1172

6,4596

6,3067

8,3368

10,702

11,129

 

S3/S1

10,465

11,061

11,38

11,574

11,730

9,1666

7,5116

10,252

12,074

13,751

 

S1

0,1758

0,1089

0,0855

0,0596

0,0465

0,0355

0,1428

0,0981

0,0728

0,0275

 

S2

1,0053

0,8198

0,7176

0,57

0,4293

0,2521

0,7832

0,729

0,6658

0,3514

 

S3

1,0706

0,9049

0,7892

0,6605

0,5357

0,3438

0,8763

0,8273

0,7576

0,4086

WB2

S2/S1

1,4873

1,2624

1,2840

1,1513

1,2033

1,1837

1,2756

1,2966

1,3386

1,1372

 

S3/S1

5,9384

5,5608

5,0313

3,6979

2,8577

2,3318

4,0252

4,7892

5,1064

3,0244

 

S1

0,0941

0,0669

0,055

0,0523

0,0414

0,0305

0,094

0,062

0,0491

0,0217

 

S2

0,1473

0,0841

0,0719

0,061

0,0504

0,0353

0,125

0,0844

0,0657

0,0248

 

S3

0,5771

0,3818

0,2885

0,2023

0,1224

0,0686

0,4152

0,3324

0,2779

0,0683

WB2

S2/S1

3,1229

2,4333

2,6135

2,0944

1,8366

1,4173

2,2195

2,3216

2,5731

1,8978

 

S3/S1

4,0662

3,3376

4,0625

3,1850

2,8014

2,1894

3,0310

3,3525

3,7237

2,9875

 

S1

0,1119

0,0671

0,054

0,0611

0,0485

0,0383

0,1052

0,0704

0,0543

0,0241

 

S2

0,3505

0,1645

0,145

0,1318

0,0914

0,0543

0,2504

0,8477

0,1515

0,0464

 

S3

0,41

0,2215

0,2269

0,1994

0,1374

0,0839

0,3213

0,8577

0,213

0,0731

  1. All protein G concentrations at each serum dilution, and all serum dilutions at each protein G concentration.
  2. WB1+WB4: Sum of wild boar 1 and wild boar 4 results. S2/S1 and S3/S1: Increase rate between pre- and post-immunization results. S1, S2 and S3: OD values.
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BMC Veterinary Research

ISSN: 1746-6148

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