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Table 4 Real and true quality SNP distribution in contigs with 4 or more ESTs

From: Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens

 

Number of contigs

Real SNPs

True SNPs

with 4 sequences

22

58

58

with 5–10 sequences

94

295

235

with 11–20 sequences

75

437

266

with 21–30 sequences

28

322

215

with 31–50 sequences

21

289

110

with > 50 sequences

17

796

274

Total

257

2197

1158

  1. Real SNPs are those which passed quality filters 1 and 2 using the pipeline QualitySNP and true SNPs are the highest quality SNPs passing the three filters (see Methods).