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Table 3 Summary statistics of SNP identification from turbot EST resources

From: Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens

 

Real SNPs

True SNPs andSNPs

Total sequences analysed

12584

Number of contigs

257

255

Total SNPs detected

2197

1158

SNP frequency

1.39/100 bp

0.74/100 bp

Total number of transitions

749

453

   C/T

556

344

   A/G

193

109

Total number of transversions

974

558

   A/T

161

87

   A/C

352

214

   T/G

251

130

   C/G

210

127

Total number of indels

366

125

Tri-allelic polymorphisms

99

21

Tetra-allelic polymorphisms

9

1

  1. Real SNPs are those which passed quality filters 1 and 2 using the pipeline QualitySNP and true SNPs are the highest quality SNPs passing the three filters (see Methods).