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Table 1 Identification of proteins from equine plasma in non-reducing 1-D SDS-PAGE. The plasma proteins were identified using LC-MS/MS.

From: Biomarkers for ragwort poisoning in horses: identification of protein targets

Band No. Protein Identification Accession No. Mr (kDa) pI MOWSE Score a (p ≤ 0.05) No. of Peptides Matched % Coverageb Species
NR1 Serum albumin CAA52194 70.6 5.95 403 (44) 41 34 Equus caballus
  Fibrinogen A α chain (fragment) AAC67561 49.6 6.45 138 (44) 11 7 Equus caballus
  Immunoglobulin gamma 4 heavy chain constant region (fragment) CAC44763 37.0 8.04 147 (44) 15 20 Equus caballus
  Immunoglobulin gamma 7 heavy chain AAS18414 36.3 7.70 109 (44) 13 - Equus caballus
  Immunoglobulin gamma 4 heavy chain AAS18415 36.2 7.71 106 (44) 15 - Equus caballus
  Transferrin AAA63684 80.3 6.83 102 (44) 5 6 Equus caballus
NR2 Serum albumin CAA52194 70.6 5.95 477 (45) 34 41 Equus caballus
  Immunoglobulin mu AAU09792 50.1 5.52 372 (45) 24 44 Equus caballus
  Lambda immunoglobulin (fragment) AAA50978 17.7 8.35 165 (45) 9 31 Equus caballus
  Fibronectin AAC48614 58.1 5.91 124 (45) 3 7 Equus caballus
NR3 Fibrinogen A α chain (fragment) AAC67561 49.6 6.45 754 (44) 42 45 Equus caballus
  Serum albumin CAA52194 68.6 5.95 265 (44) 22 24 Equus caballus
  Fibrinogen γ B chain CAA33562 50.8 5.54 121 (44) 6 6 Bos taurus
NR4 Lamba Immunoglobulin (fragment) AAA50978 17.4 8.35 274 (44) 15 32 Equus caballus
  Immunoglobulin gamma 5 heavy chain constant region (fragment) CAC86340 35.9 5.95 272 (44) 20 28 Equus caballus
  Immunoglobulin gamma 1 heavy chain constant region (fragment) CAC44624 37.4 7.68 270 (44) 7 17 Equus caballus
  Serum albumin CAA52194 68.6 5.95 265 (44) 22 24 Equus caballus
NR5 Immunoglobulin gamma 1 heavy chain constant region (fragment) CAC44624 37.4 7.68 554 (44) 26 34 Equus caballus
  Immunoglobulin G heavy chain (fragment) AAG01011 47.6 5.70 411 (44) 30 27 Equus caballus
NR6 Serum albumin CAA52194 70.6 5.95 717 (44) 59 48 Equus caballus
  Immunoglobulin gamma 4 heavy chain AAS18415 36.2 7.71 463 (44) 59 - Equus caballus
  Immunoglobulin gamma 7 heavy chain AAS18414 36.3 7.70 454 (44) 56 - Equus caballus
  Immunoglobulin gamma 4 heavy chain constant region (fragment) CAC44763 37.0 8.04 374 (44) 46 37 Equus caballus
  Lambda immunoglobulin (fragment) AAA50978 7.7 8.35 337(44) 52 - Equus caballus
NR7 Gelsolin Q28372 80.9 5.58 354(44) 15 20 Equus caballus
NR8 Transferrin AAA63684 80.3 6.83 421 (44) 31 27 Equus caballus
  Carboxylesterase 1 AAH21150 63.0 5.64 114 (44) 6 6 Mus musculus
NR9 Transferrin AAA63684 80.3 6.83 665 (44) 51 32 Equus caballus
  α-1-antitrypsin AAC83412 47.1 5.23 150 (44) 7 8 Equus caballus
  Serum albumin AAV28861 70.5 5.89 95 (44) 8 12 Equus asinus
  1. Proteins were identified on the basis of their MOWSE score using the MSDB database. Each band number refers to proteins identified in Figure 2A. a = MOWSE baseline significance score in brackets; b = for those with no percentage coverage value the database did not give this information.