Monitoring the effect of pyrrole exposure on equine plasma fibrinogen using western blotting. Equine plasma was reacted with varying concentrations of DHM for 60 min at room temperature. Control plasma was treated with a reaction mixture that was complete apart from DHM. Samples were analysed by 1-D SDS-PAGE and proteins were blotted onto nitrocellulose membranes and blocked overnight before probing with an anti-equine fibrinogen antibody. Blots were then incubated with an alkaline phosphatase linked secondary antibody and developed. Migration of fibrinogen α-chain was evident following the exposure of a large excess of pyrrole.