The effect of pyrrole exposure on equine plasma protein profile. Equine plasma was reacted with 10–100 molar excess of DHM for 60 min at room temperature. Control horse plasma was treated with a reaction mixture that was complete apart from DHM. Proteins were separated by 1-D SDS-PAGE on 7.5% (w/v) gels under (A) non-reducing and (B) reducing conditions. Gels were stained with Coomassie brilliant blue. High molecular weight aggregates are evident in pyrrole treated lanes from both gels. Proteins identifications are detailed in Tables 1 and 2.