From: Prevalence of the prion protein gene E211K variant in U.S. cattle
Primer numbera | DNA sequenceb | Gene region | Orientation | Function | Tm (°C) | STS length (bp) | Termination mixture | Mass (Daltons) | Codons 210/211c |
---|---|---|---|---|---|---|---|---|---|
67661 | gac att aga atc act tcc ata gg | Intron 2, CDS, 3' UTR | Sense | Amplification, sequencing | 58 | 1498 | - d | - | - |
65547 | ata ctg agc taa cgg gac tt | Intron 2, CDS, 3' UTR | Antisense | Amplification, sequencing | 58 | 1498 | - | - | - |
65617 | gat ttt tac atg ggc ata tga | Intron 2, CDS, 3' UTR | Sense | Amplification, sequencing | 60 | 985 | - | - | - |
42618 | gcc aag ggt att agc ata ca | Intron 2, CDS, 3' UTR | Antisense | Amplification, sequencing | 60 | 985 | - | - | - |
42642 | cct gga ggc aac cgt tat | CDS | Sense | Sequencing | 62 | - | - | - | - |
27774 | ctc ctg cca cat gct tca t | CDS | Antisense | Sequencing | 62 | - | - | - | - |
58117 | tac agg cca gtg gat cag ta | CDS | Sense | Amplification | 60 | 203 | - | - | - |
58119 | gcc cct cgt tgg taa taa | CDS | Antisense | Amplification | 60 | 203 | - | - | - |
58118 | acg ttg gat gta cag gcc agt gga tca gta | CDS | Sense | Amplification for hME (with mass tag) | 60 | 226 | - | 9311.10 | - |
58120 | tgg acg ttg gat ggc ccc tcg ttg gta ata a | CDS | Antisense | Amplification for hME (with mass tag) | 60 | 226 | - | 9582.20 | - |
58141 | caccaagggggagaacttcac-C-G-aaactgacatcaagatgat | CDS | Sense | control template for cE211 allele | 93 | - | - | 12958.40 | - |
58142 | atcatcttgatgtcagttt-C-G-gtgaagttctcccccttggtg | CDS | Antisense | control template for cE211 allele | 93 | - | - | 12868.30 | - |
64161 | caccaagggggagaacttcac-T-G-aaactgacatcaagatgat | CDS | Sense | control template for tE211 allele | 93 | - | - | 12973.50 | - |
64162 | atcatcttgatgtcagttt-C-A-gtgaagttctcccccttggtg | CDS | Antisense | control template for tE211 allele | 93 | - | - | 12852.30 | - |
58143 | caccaagggggagaacttcac-C-A-aaactgacatcaagatgat | CDS | Sense | control template for cK211 allele | 93 | - | - | 12942.40 | - |
58144 | atcatcttgatgtcagttt-T-G-gtgaagttctcccccttggtg | CDS | Antisense | control template for cK211 allele | 93 | - | - | 12883.30 | - |
58145 | caccaagggggagaacttcac-T-A-aaactgacatcaagatgat | CDS | Sense | control template for tK211 allele | 93 | - | - | 12957.50 | - |
58146 | atcatcttgatgtcagttt-T-A-gtgaagttctcccccttggtg | CDS | Antisense | control template for tK211 allele | 93 | - | - | 12867.30 | - |
62792 | caa ggg gga gaa ctt cac | CDS | Sense | hME primer | 58 | - | ddAe | 5557.60 | - |
- | caa ggg gga gaa ctt cac ca | CDS | Sense | CA allele analyte | - | - | ddA | 6144.00f | cK211 |
- | caa ggg gga gaa ctt cac ta | CDS | Sense | TA allele analyte | - | - | ddA | 6159.00f | tK211 |
- | caa ggg gga gaa ctt cac cga | CDS | Sense | CGA allele analyte | - | - | ddA | 6473.20f | cE211 |
- | caa ggg gga gaa ctt cac tga | CDS | Sense | TGA allele analyte | - | - | ddA | 6488.20f | tE211 |
58148 | atc atc ttg atg tca gtt t | CDS | Antisense | hME primer | 49 | - | ddC/ddGe | 5783.80 | - |
- | atc atc ttg atg tca gtt tc | CDS | Antisense | C allele analyte | - | - | ddC/ddG | 6057.00 | nE211 |
- | atc atc ttg atg tca gtt ttg | CDS | Antisense | TG allele analyte | - | - | ddC/ddG | 6401.20 | cK211 |
- | atc atc ttg atg tca gtt tta g | CDS | Antisense | TAG allele analyte | - | - | ddC/ddG | 6714.40 | tK211 |