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Table 1 Akabane virus strains isolated during an outbreak of bovine encephalomyelitis and used in this study

From: Bovine epizootic encephalomyelitis caused by Akabane virus in southern Japan

   

Source

   

Strain

Passage levela

Geographical origin

    
   

Specimen for isolation

Date collected

Symptom

Profile of cattle

KM-1/Br/06

Sm1, HL4

Kumamoto

Brain

3 October, 2006

Astasia, Tachypnea

Holstein, 4 days old

KM-2/Br/06

HL4

Kumamoto

Brain

28 September, 2006

Astasia, Tachypnea, Tremor, Opisthotonus

Holstein, female, 4 months old

KM-3/Ce/06

HL3

Kumamoto

Cerebellum

19 October, 2006

Astasia

Holstein, 5 months old

KM-6/Br/06

HL3

Kumamoto

Brain stem

26 October, 2006

Astasia

Holstein, female, 19 months old

KM-7/Pt/06

HL4

Kumamoto

Spinal cord (Pars thoracia)

26 October, 2006

Astasia

Holstein, female, 19 months old

KM-8/Br/06

HL4

Kumamoto

Brain stem

18 October, 2006

Astasia

Holstein, female, 16 months old

KM-9/B/06

HL3

Kumamoto

Blood

30 October, 2006

No symptoms were observed when a blood sample was taken. Astasia appeared on 13 November, 2006.

Holstein, 3 months old

KM-10/B/06

HL3

Kumamoto

Blood

September, 2006

No symptoms

 

KM-11/B/06

HL2

Kumamoto

Blood

September, 2006

No symptoms

 

KS-1/P/06

HL4

Kagoshima

Plasma

12 October, 2006

No symptoms

Japanese Black, castrated male, 10 months old

KS-2/Mo/06

HL5

Kagoshima

Brain stem (Medulla oblongata)

18 October, 2006

Dysstasia, Ataxia

Japanese Black, castrated male, 6 months old

KSB-3/P/06

BHK4

Kagoshima

Plasma

27 October, 2006

No symptoms

Japanese Black, female, 24 months old

  1. a Letters indicate cell lines and numbers indicate number of passages. HL, HmLu-1 cells; BHK, BHK-21 cells; Sm, suckling-mouse brain (only for isolation of KM-1/Br/06).