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Table 2 Primer characteristics [12] and calculated % diversity indices for each of the tandem repeats (TRs).

From: Multiple-locus variable number of tandem repeat analysis (MLVA) of Irish verocytotoxigenic Escherichia coliO157 from feedlot cattle: uncovering strain dissemination routes

Primer Sequence 5' to 3' Size [mer] Tm °C TR Sequence TR size *Diversity Index (%)
TR 1 -ACT GCA TGA TAA GCC TCA GG- 20     
  -CAC TGA AGC CTG TTC CGT TC- 20 57 -AAATAG- 6 77.0
TR 2 -CGC AGT TGA TAC CTA CGG - 18     
  -GGA AGG AAG CTG ATA GGT - 18 53 -TGGCTC- 6 92.2
TR 3 -TCT TGT CAA TAT AGA TTG G- 19     
  -TGA TTA AGC GTG TAC TGA - 18 50 -TATCTT- 6 77.6
TR 4 -GGT GAT GGC TTG ATA TTG A- 19     
  -GCC ACA CTG CGA GTA TAG AG- 20 53 -TGCAAA - 6 66.5
TR 5 -GTT GAT TAT CAT GGT ATG TC- 20     
  -GGA CAA CTT GTA GTA CAA G- 19 51 -AAGGTG - 6 66.1
TR 6 -GAT GGT TCG ACT AAC CGT TAT- 21     
  -TAG CAG ATG TTC GTT CCT- 18 53 -TTAAATAATCTACAGAAG - 18 55.9
TR 7 -CGC AGT GAT CAT TAT TAG C- 19     
  -TGC TGA AAC TGA CGA CCA GT- 20 50 -GACCAC- 6 71.3
  1. * Diversity is based on Simpson's index calculated using all fifty-four isolates