Figure 6
Immunoblot analyses of S100A12 and PTX3 expression in milk whey from S. aureus infected cattle. Representative immunoblot analyses of milk whey proteins before and after infection of mammary tissue with S. aureus. Each sample comprised 25 μl of milk whey and was run under reducing conditions. (a) Representative analysis of S100A12 expression in milk whey from cow 1419. Lane 1, control quarter pre-infection; lane 2, control quarter post-infection; lane 3, pre-infection control from the quarter receiving 1 × 105 S. aureus; lane 4, post-infection sample (1 × 105 bacteria); lane 5, pre-infection control from the quarter receiving 1 × 106 S. aureus; lane 6, post-infection sample (1 × 106 bacteria). Arrows denote the positions of S100A12 immunoreactive oligomers (monomer, dimer, trimer) detected using the immunoaffinity purified antibodies raised to rS100A12. (b) Analysis of PTX3 expression in milk whey. Lane 1, cow 1419 pre-infection sample from the quarter receiving 1 × 105 S. aureus; lane 2, post-infection sample (1 × 105 bacteria); lane 3, cow 1419 pre-infection sample from the quarter receiving 1 × 106 S. aureus; lane 4, cow 1419 post-infection sample (1 × 106 bacteria); lane 5, cow 1490 pre-infection sample from the quarter receiving 1 × 105 S. aureus; lane 6, cow 1490 post-infection sample (1 × 105 bacteria); lane 7, cow 1592 pre-infection control from the quarter receiving 1 × 105 S. aureus; lane 8, cow 1592 post-infection sample (1 × 105 bacteria). Arrows denote the positions of PTX3 immunoreactive bands (monomer and dimer) detected using immunoaffinity purified antibodies raised to rPTX3.