Time-courses of S100A12 and PTX3 expression in bMEC stimulated with LPS and LTA. bMEC were stimulated with either 50 μg/ml LPS (■) or 20 μg/ml LTA (⚏) and the expression levels of S100A12 (a) and PTX3 (b) were monitored using qRT-PCR over a 24 h period. Data are expressed as mean fold change (n = 3) compared to expression in unstimulated cells. Significance was determined by ANOVA compared to unstimulated bMEC. P < 0.01 (*) was considered significant.