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Table 5 Sequences for primers and probes for candidate reference genes used in the study (5'-3' direction).

From: Expression stability of commonly used reference genes in canine articular connective tissues

Gene

Forward Primer

Reverse Primer

Probe

Amplicon Length Bp

Efficiency of Assay (%age)

Accession Number for sequence used

GAPDH

CTGGGGCTCACTTGAAAGG

CAAACATGGGGGCATCAG

CTGCTCCT

72

102.3

NM_001003142

ACTB

CCCATCTACGAGGGGTACG

GGATCTTCATGAGGTAGTCAGTCA

CTGGCTGG

88

101.9

NM_001003349

B2M

CCTTGCTCCTCATCCTCCT

TGGGTGTCGTGAGTACACTTG

CAGCATCC

83

99.5

XM_535458

HMBS

GCATGTATGCTGTGGGTCAG

GCATGTCCTGGTCCTTGG

GGCCCTGG

63

101.0

XM_546491

HPRT

TGTGCTCAAGGGAGGCTATAA

CCATCACTATTTCTGTTCAGTGCT

ACCTGCTG

75

101.8

NM_001003357

RPL13A

CTGCCCCACAAGACCAAG

GGGATCCCATCAAACACCT

CCAGGCTG

65

98.6

ENSCAFG00000003633

SDHA

GGTGGCACTTCTACGACACC

ATGTAGTGGATGGCGTCCTG

CTGGCTGG

67

102.5

XM_535807

TBP

TCCACAGCCTATCCAGAACA

CTGCTGCTGTTGTCTCTGCT

CTGGAGGA

67

97.4

ENSCAFG00000004119

YWHAZ

GTTACTTGGCCGAAGTTGCT

TGCTTGTTGTGACTGATCCAC

TGGTGATG

65

100.7

XM_532287