Figure 2

Amino acid changing polymorphisms in two areas of the porcine desmoglein 1 gene. A, Degenerate sequences of the porcine desmoglein 1 in the investigated regions showing part of the fragments amplified in the genotyping procedure. SNPs are marked with X. R: A or C, W: A or T, M: A or C, Y: C or T. B, Amino acid sequence alignment of human (HUMAN)DSG1 GenBank: AAC83817 and porcine (PIG)DSG1 GenBank: DQ823081 desmoglein 1 in the region around 110 amino acids upstream of the cleavage site. Underlined in the human sequence are the amino acids shown to be necessary for cleavage by exfoliative toxin ETA from S. aureus. Marked with asterisks * in the porcine sequence are the residues changed by single nucleotide polymorphisms found in this study. C, Alignment of (HUMAN)DSG1 and (PIG)DSG1 around the cleavage site (marked with arrow). Marked with asterisk * in the porcine sequence is the amino acid changed by single nucleotide polymorphism.