Figure 2
From: Relevance of the regional lymph node in scrapie pathogenesis after peripheral infection of hamsters
Western blot detection of PrP27–30, the Proteinase K-resistant core of PrPTSE, in lymphonodal tissue (Ln. popliteus) at different time points after infection of hamsters via the footpad with a 2% 263K-scrapie brain homogenate. The amount of tissue subjected to testing is specified in brackets. A, left lane: blot control, 263K-scrapie hamster brain homogenate containing 10-6 g of brain tissue; right lane: 27kD marker; B, 2 dpi, left lane: contralateral Ln. (3.1 mg); right lane: ipsilateral Ln. (3.3 mg); C, 42 dpi, left lane: contralateral Ln. (2.9 mg); right lane: ipsilateral Ln.(4.8 mg); D, 70 dpi, left lane: contralateral Ln. (7.0 mg); right lane: ipsilateral Ln. (7.4 mg); E, 80 dpi, left lane: contralateral Ln. (7.9 mg); right lane: ipsilateral Ln. (4.7 mg); F, 90 dpi, left lane: contralateral Ln. (4.1 mg); right lane: ipsilateral Ln. (4.3 mg); G, 100 dpi, left lane: contralateral Ln.(1.9 mg); right lane: ipsilateral Ln.(3.9 mg).