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Table 3 Results from trial 1–3; poultry faecal samples artificially contaminated with salmonella. Faeces originating from two egg-producing farms (Trial 1), two egg-producing farms and one broiler farm (Trial 2–3). For each trial different spiking levels of salmonella were constructed (Trial 1 S. Enteritidis, Trial 2 S. Livingstone, Trial 3 S. Worthington), each spiking level containing six 25 g samples of faeces.

From: Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

CFU salm/25 g NMKL MSRV MSRV PCR VIDAS SLM SELECTA ELISA SELECTA CULTURE OPTIMA ELISA OPTIMA CULTURE BAX® SELECTA PCR SELECTA IMS PCR
TRIAL 1            
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
5000 6/6 6/6 6/6 6/6 6/6 6/6 0/6 6/6 6/6 6/6 6/6
500 4/6 6/6 6/6 6/6 6/6 6/6 0/6 4/6 6/6 6/6 0/6
50 0/6 6/6 6/6 0/6 6/6 6/6 0/6 1/6 6/6 26 0/6
TRIAL 2            
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
13,000 6/6 6/6 6/6 6/6 6/6 6/6 1/6 6/6 6/6 6/6 6/6
1300 6/6 6/6 6/6 2/6 1/6 6/6 0/6 6/6 6/6 6/6 0/6
130 3/6 6/6 6/6 0/6 0/6 6/6 0/6 2/6 4/6 2/6 0/6
13 1/6 6/6 6/6 0/6 0/5*** 4/5*** 0/6 0/6 4/6 0/5 *** 0/5***
TRIAL 3            
0 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6 0/6
8000 6/6 6/6 6/6 6/6 0/6 6/6 0/6 6/6 6/6 6/6 5/6
800 6/6 6/6 6/6 0/6 0/6 6/6 0/6 6/6 6/6 6/6 4/6
80 6/6 6/6 6/6 0/6 0/6 6/6 0/6 6/6 6/6 6/6 1/6
8 6/6 6/6 6/6 0/6 0/6 6/6 0/6 0/6 6/6 0/6 0/6
TP* 50 68 68 26 25 64 1 43 62 50 40
TN* 18 18 18 18 18 18 18 18 18 18 18
FP* 0 0 0 0 0 0 0 0 0 0 0
FN* 16 0 0 40 40 1 65 23 4 15 26
AC** 0.8 1.0 1.0 0.52 0.52 0.99 0.23 0.73 0.95 0.82 0.69
SE** 0.76 1.0 1.0 0.4 0.38 0.98 0.02 0.65 0.94 0.77 0,61
SP** 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
  1. *TP = True positives, TN = True negatives, FP = False positives, FN = False negatives
  2. **AC = Accuracy, SE = Sensitivity, SP = Specificity
  3. *** Due to a technical fault, one of the sextuplicates in the lowest spiking level was excluded for the Selecta enrichment based methods.