Skip to main content

Table 3 Results from trial 1–3; poultry faecal samples artificially contaminated with salmonella. Faeces originating from two egg-producing farms (Trial 1), two egg-producing farms and one broiler farm (Trial 2–3). For each trial different spiking levels of salmonella were constructed (Trial 1 S. Enteritidis, Trial 2 S. Livingstone, Trial 3 S. Worthington), each spiking level containing six 25 g samples of faeces.

From: Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

CFU salm/25 g

NMKL

MSRV

MSRV PCR

VIDAS SLM

SELECTA ELISA

SELECTA CULTURE

OPTIMA ELISA

OPTIMA CULTURE

BAX®

SELECTA PCR

SELECTA IMS PCR

TRIAL 1

           

0

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

5000

6/6

6/6

6/6

6/6

6/6

6/6

0/6

6/6

6/6

6/6

6/6

500

4/6

6/6

6/6

6/6

6/6

6/6

0/6

4/6

6/6

6/6

0/6

50

0/6

6/6

6/6

0/6

6/6

6/6

0/6

1/6

6/6

26

0/6

TRIAL 2

           

0

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

13,000

6/6

6/6

6/6

6/6

6/6

6/6

1/6

6/6

6/6

6/6

6/6

1300

6/6

6/6

6/6

2/6

1/6

6/6

0/6

6/6

6/6

6/6

0/6

130

3/6

6/6

6/6

0/6

0/6

6/6

0/6

2/6

4/6

2/6

0/6

13

1/6

6/6

6/6

0/6

0/5***

4/5***

0/6

0/6

4/6

0/5 ***

0/5***

TRIAL 3

           

0

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

0/6

8000

6/6

6/6

6/6

6/6

0/6

6/6

0/6

6/6

6/6

6/6

5/6

800

6/6

6/6

6/6

0/6

0/6

6/6

0/6

6/6

6/6

6/6

4/6

80

6/6

6/6

6/6

0/6

0/6

6/6

0/6

6/6

6/6

6/6

1/6

8

6/6

6/6

6/6

0/6

0/6

6/6

0/6

0/6

6/6

0/6

0/6

TP*

50

68

68

26

25

64

1

43

62

50

40

TN*

18

18

18

18

18

18

18

18

18

18

18

FP*

0

0

0

0

0

0

0

0

0

0

0

FN*

16

0

0

40

40

1

65

23

4

15

26

AC**

0.8

1.0

1.0

0.52

0.52

0.99

0.23

0.73

0.95

0.82

0.69

SE**

0.76

1.0

1.0

0.4

0.38

0.98

0.02

0.65

0.94

0.77

0,61

SP**

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

1.0

  1. *TP = True positives, TN = True negatives, FP = False positives, FN = False negatives
  2. **AC = Accuracy, SE = Sensitivity, SP = Specificity
  3. *** Due to a technical fault, one of the sextuplicates in the lowest spiking level was excluded for the Selecta enrichment based methods.