Table 2 Results from 100 pooled samples consisting of assumes Salmonella-free faeces. Assumed salmonella-free faecal samples originating from 265 farms (poultry, pig, and cattle) were analysed as 100 pooled samples, each consisting of 25 g of faeces; no salmonella bacteria added. In parallel, three control samples, spiked with 300 cfu Salmonella Typhimurium DT1, 2800 cfu Salmonella Worthington, and 5000 cfu Salmonella Typhimurium DT40 respectively, were analysed.
From: Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry
CFU salm/25 g | NMKL | MSRV | MSRV PCR | VIDAS SLM | SELECTA ELISA | SELECTA CULTURE | OPTIMA ELISA | OPTIMA CULTURE | BAX® | SELECTA PCR | SELECTA IMS PCR |
---|
0
| 0/100 | 0/100 | 0/100 | 0/100 | 0/100 | 0/100 | 0/100 | 0/100 | 1/100 | 0/100 | 0/100 |
Control
| 3/3 | 3/3 | 3/3 | 2/3 | 3/3 | 3/3 | 2/3 | 2/3 | 3/3 | 3/3 | 3/3 |
TP*
| 3 | 3 | 3 | 2 | 3 | 3 | 2 | 2 | 3 | 3 | 3 |
TN*
| 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 99 | 100 | 100 |
FP*
| 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
FN*
| 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
AC**
| 1.0 | 1.0 | 1.0 | 0.99 | 1.0 | 1.0 | 0.99 | 0.99 | 0.99 | 1.0 | 1.0 |
SE**
| 1.0 | 1.0 | 1.0 | 0.67 | 1.0 | 1.0 | 0.67 | 0.67 | 1.0 | 1.0 | 1.0 |
SP**
| 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 0.99 | 1.0 | 1.0 |
- *TP = True positives, TN = True negatives, FP = False positives, FN = False negatives
- **AC = Accuracy, SE = Sensitivity, SP = Specificity