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Table 2 Results from 100 pooled samples consisting of assumes Salmonella-free faeces. Assumed salmonella-free faecal samples originating from 265 farms (poultry, pig, and cattle) were analysed as 100 pooled samples, each consisting of 25 g of faeces; no salmonella bacteria added. In parallel, three control samples, spiked with 300 cfu Salmonella Typhimurium DT1, 2800 cfu Salmonella Worthington, and 5000 cfu Salmonella Typhimurium DT40 respectively, were analysed.

From: Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry

CFU salm/25 g NMKL MSRV MSRV PCR VIDAS SLM SELECTA ELISA SELECTA CULTURE OPTIMA ELISA OPTIMA CULTURE BAX® SELECTA PCR SELECTA IMS PCR
0 0/100 0/100 0/100 0/100 0/100 0/100 0/100 0/100 1/100 0/100 0/100
Control 3/3 3/3 3/3 2/3 3/3 3/3 2/3 2/3 3/3 3/3 3/3
TP* 3 3 3 2 3 3 2 2 3 3 3
TN* 100 100 100 100 100 100 100 100 99 100 100
FP* 0 0 0 0 0 0 0 0 1 0 0
FN* 0 0 0 1 0 0 1 1 0 0 0
AC** 1.0 1.0 1.0 0.99 1.0 1.0 0.99 0.99 0.99 1.0 1.0
SE** 1.0 1.0 1.0 0.67 1.0 1.0 0.67 0.67 1.0 1.0 1.0
SP** 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 0.99 1.0 1.0
  1. *TP = True positives, TN = True negatives, FP = False positives, FN = False negatives
  2. **AC = Accuracy, SE = Sensitivity, SP = Specificity