Western immunoblot of cases 05/377 and 06/008. Samples from 05/377 and 06/008 are shown in duplicate lanes; a diluted sample of case 05/377 was included to show the different bands more clearly. Controls are shown in single lanes and comprise a negative control sample (Suffolk, ARQ/ARQ), a known UK classical scrapie case (Poll Dorset, VRQ/VRQ), a known UK bovine BSE case and a known Nor98 Norwegian ovine scrapie case (Norwegian White, AHQ/AHQ). Molecular mass markers were included at either end of the gel (ECL DualVue, Amersham Biosciences, Little Chalfont, UK). The antibody used to detect PrPres in the test kit is Sha31 .