Table 1 Evaluation of markers. ++: good staining (strong, specific), +: poor staining (weak or including non-specifically stained cells), -: unsuccesful staining. PFA: paraformaldehyde fixation, EtOH: ethanol fixation. N: no epitope retrieval, P: protease-induced epitope retrieval, H3: heat-induced epitope retrieval (HIER) at pH 3, H6: HIER at pH 6, H9: HIER at pH 9.5. Methods: A = avidin-biotin-complex, T = tyramide signal amplification. NF = neurofilament. Sources: DHSB = Developmental Studies Hybridoma Bank, BD = BD Biosciences, N/L = NeoMarkers/LabVision, Vector = Vector Laboratories, W/H = Witten/Herdecke University, Ch = Chemicon, SM = Sternberger Monoclonals, b/C = bAbco/Covance, VMRD: Veterinary Medicine Research Diagnostics, SC = Santa Cruz, BL = Bethyl Laboratories, Bm = Biomeda, CST = Cell Signaling Technology.

EPITHELIUM, ENDOTHELIUM

collagenType IV

M3F7

human collagen

DSHB

[10]

1:200T

cytokeratin, HMW

34betaE12

human epid. keratin

Dako

[5]

1:1000T

cytokeratin, pan

rabbit polycl.

bovine epid. keratin

Zymed

n.a.

1:1000T

eNos/NOSType III

rabbit polycl.

peptide (human)

BD

[11]

1:1000T

keratin, pan

AE1 + AE3

human epid. keratin

N/L

[2,3]

1:100A, 1:1000T

keratin, pan

Lu-5

lung cancer cell line

N/L

[4]

1:100A, 1:1000T

lectin GSL I-B4

G. simplicifolia

n.a.

Vector

[8,9]

1:4000T

lectin ML-I

Viscum album

n.a.

W/H

[7]

1:2000T

von Willebrand

rabbit polycl.

human vWF

Dako

[6]

1:400A, 1:800T

CONNECTIVE TISSUE

procollagenType I

SP1.D8

ovine aminopropept.

DSHB

[14]

1:2000T

vimentin

V9

porcine vimentin

Dako

[12,13]

1:100A, 1:500T

MUSCLE

actin, muscle

HHF35

n.a.

Enzo

[17]

1:100A

actin, smooth muscle α

1A4

peptide

Dako

[18]

1:500T

desmin

D33

human desmin

Dako

[15,16]

1:400A, 1:2000T

NEURONAL TISSUE

CNPase

11-5B

human brain CNPase

Ch

n.a.

1:400A

GFAP

rabbit polycl.

human GFAP

Zymed

n.a.

1:200A

NeuN

A60

mouse neuronal nuclei

Ch

[19,22]

1:4000T

NF 160/200 kD

RMdO-20

rat neurofilaments

Zymed

[22]

1:400A

NF, pan

SMI311 (cockt)

n.a.

SM

[21]

1:1000A, 1:2000T

O4

81

bovine brain

Roche

[23]

1:2A

S100

rabbit polycl.

bovine S100

Dako

[24]

1:800A

tubulin βIII

rabbit polycl.

peptide (rat)

b/C

n.a.

1:6000A,1:10000T

tubulin βIII

TU-20

n.a.

Ch

[20]

1:400A, 1:3000T

LEUKOCYTES

CD11a/18

MUC76A

sheep, pig leukocytes

VMRD

[28]

1:40A

CD11a/18

BAT75A

ruminant leukocytes

VMRD

[27]

1:40A, 1:200T

CD11b

MM10A

n.a.

VMRD

[32]

1:500T

CD14

MM61A

bovine mononucl. cells

VMRD

[25]

1:200

CD34

rabbit polycl.

peptide (human)

SC

n.a.

1:200A, 1:200T

CD3ε

rabbit polycl.

peptide (human)

Dako

[29]

1:100A, 1:1000T

CD45

CACTB51A

bovine act. lymphocytes

VMRD

[25]

1:800T

CD45

CC1

n.a.

Serotec

[26]

1:100A

CD68

EBM11

human macrophages

Dako

[33]

1:80A

CD79αcy

HM57

peptide (human)

Dako

[30]

1:500T

IgA

rabbit polycl.

bovine IgA

BL

n.a.

1:100A

IgM

rabbit polycl.

bovine IgM

BL

n.a.

1:1500A

IgM

BIg73A

bovine Ig

VMRD

[31]

1:5000A

lysozyme

rabbit polycl.

human lysozyme

Bm

[34]

1:500T

CELL STATUS

cleaved caspase 3 (Asp175)

rabbit polycl.

peptide (human)

CST

[37]

1:75A

Ki67 antigen

MIB-1

peptide (human)

Dako

[35,36]

1:4000T