Table 2 Primers, and gene accession number, annealing temperature, and product size for quantitative real-time PCR analysis
Gene | Forward & Reverse primer | Accession no | (°C) | (bp) |
|---|---|---|---|---|
GAPDH | CCTGGAGAAACCTGCCAAGT GCCAAATTCATTGTCGTACCA | TC186924 | 58 | 226 |
RPLPO | CAACCCTGAAGTGCTTGACAT AGGCAGATGGATCAGCCA | TC204704 | 62 | 220 |
CD14 | TGAACATTGCCCAAGCACAC GCCGAGACTGGGATTGTCAG | AY289201 | 58 | 101 |
C3 | AGAAGCAGAAGCCTGATGGA CCTCGCAGATGTCTTTAGCC | AF038130 | 60 | 150 |
IFNaR | CGCATAAGAGCAGAAGAAGGA TGTTCCAGGGGGAGAGATG | U65978 | 60 | 150 |
NRAMP1 | TTGGCACAGCTATTGCATTC TTCCGCAACCCGTAGTTATC | AF005380 | 60 | 121 |
SOD | CGAGGCAAAGGGAGATACAG TCTCCAAACTGATGGACGTG | M81129 | 60 | 90 |
MCP-1 | GGGTGCTCATTCAGACCATT CATGGGAACTCAAGGAGGAA | L32659 | 60 | 126 |
IGFBP3 | CAGAGCACAGACACCCAGAA TGCCCGTACTTATCCACACA | AF305199 | 58 | 233 |