Table 2 Primers used in this study
From: Evaluation of tandem repeats for MLVA typing of Streptococcus uberis isolated from bovine mastitis
VNTR | Primer sequence 5'-3' | Location in 0140J strain genome |
|---|---|---|
01–02 | TGCAGCACAAATGGAAACTGC | 26778–26798 |
AGCTACAGGTGCAGGTGTTGC | 27001–27021 | |
03 | CAGAATTAAAATATCCTTGTTTTTAC | 37728–37753 |
ATTCTTCTTCATAGTCATATAGTGGT | 37942–37967 | |
04 | TGAAACTGCAACACGTACAGG | 73293–73313 |
ACGTTTGGAGTTGCTTCGATG | 73709–73729 | |
05 | CGCAGAAAATCCTCAAGGAGC | 78991–79011 |
ACAGCATCACCAACACCCATG | 79290–79310 | |
06–07 | CATATTACCTCTTATTCCTCTT | 167562–167583 |
TCAATAATATCAACAATCTCA | 168054–168074 | |
08 | TTTGGATGCTTTCTTGGTTGG | 242005–242025 |
TTGCAGAGATTCGCCAACCTG | 242325–242345 | |
09 | CTTTTAGCATAATTTTACCTTTTA | 281297–281320 |
ATAAGATCAATATGAAGTGTATCTA | 281591–281615 | |
10 | CGAACATGGTTATATCGTCCCAG | 423092–423114 |
TCGGCAACAATGGCCATATCG | 423980–424000 | |
11–12 | GTTTATTATCTTTATGAGTATGCTT | 591027–591051 |
TATCTATAAAATGCAAACGATTTA | 591969–591992 | |
13 | ATGAACCGACAGCTAGTCTGG | 654229–654249 |
CATATGGTCGTACGGTCCCTG | 654572–654592 | |
14 | CAAGCCTTGAAATTCATCTCC | 902092–902112 |
GCAAGGTTTTTCCTCGTTGA | 902638–902657 | |
15 | AATATAGGAATTAATTTCAGTTCTTGAC | 991780–991807 |
TAAGTTTACCCTAGAAGAGAATACAGTT | 992388–992415 | |
16–17 | ACCATTAGAGATGGTTACTTCTTTAC | 1066796–1066821 |
GTCTGAACGGACTTAATGGTATT | 1067309–1067331 | |
18 | GAAACAAAGTTTCCATCTTTAT | 1136358–1136379 |
AAGATGAAGTTTATTATGGATTCTC | 1137131–1137155 | |
19 | CCTCCACCTGATGGACCAACG | 1146876–1146896 |
CAGGTTGTCGAAGCAACTGTC | 1147205–1147225 | |
20 | AAAAGAACTTTATGAATGGAAAAC | 1209150–1209173 |
ATGGTATCTAATAAGGTAAGACCAC | 1210065–1210089 | |
21 | CAGTTGTACTTGTGGCTTGAC | 1240077–1240097 |
TTGCCCTTCGTTGGTTCGCTG | 1240581–1240601 | |
22 | CCCCTAAAATCGCAGCAATA | 1345744–1345763 |
TTTGACCAATTATGGTCTATACGC | 1345908–1345931 | |
23 | TTTACCTTCAGTTTCTTTAATC | 1494658–1494679 |
TAAAGATTTAGGTACTCAAAAAG | 1494840–1494862 | |
24 | ACTTCCGAATTGTCATCGTTCG | 1496796–1496817 |
TGCTGACAGTAATGAACCAGC | 1497170–1497190 | |
25 | GGTGTTTATTTCATATTGGTCAGC | 1813638–1813661 |
GAAGTTCAAAGTTGGTTCAAATGTT | 1813932–1813956 |