Representative agarose gel electrophoresis of PCR-amplified VNTR. Lane M1: 100 bp DNA ladder; lane M2: 100 bp+500 bp DNA ladder. Numbers on top of other lanes designate alleles expressed as number of repeats, except for TR11+12. According representative amplicons sequences of TR11+12, alleles 1 to 5 corresponded to 2 TR12, 1 TR11 and 1 TR12, 1 TR11 and 2 TR12, 3 TR11 and 1 TR12, 4 TR11 and 2 TR12, respectively. The predicted size in bp of alleles, calculated by sequencing of representative amplicons and S. uberis 0140J genome sequence data, is indicated. Nd: the predicted size of this allele was not calculated because the sequence of the corresponding amplicon was incomplete.