Muscle gene expression in P311 -adenovirus infected C2C12 cells. Real-time PCR was performed on cDNAs derived from infected cells over a time course as detailed in Fig. 4. No dramatic difference in MYH expression between P311-infected and control C2C12 cells was seen during the early infection time points (3I and 3I 3D). Reduction in expression of MYHembryonic, MYH2b, Myf-5 and α-actin in P311-infected C2C12 myotubes was noticeable at the last time point (3I 6D). Results expressed as mean ± standard deviation from triplicate samples within the same experiment.