Effect of calcineurin over-expression on endogenous P311 expression in C2C12 cells. (A) Cells were infected with a constitutively active calcineurin (Cn) or GFP-control adenoviral construct in PM for 2 days (2I), or for 3 days (3I), followed by 1 day DM (3I 1D), or 3 days in DM (3I 3D), or 6 days in DM (3I 6D). (B) Cells were also infected as myotubes (at 4 days in DM) for 2 days, followed by a further 2 days in DM (4+4), or 5 days in DM (4+7), or 7 days in DM (4+9). In both series (A and B), extended expression of Cn led to a reduction of P311 expression. Results expressed as mean ± standard deviation from triplicate samples within the same experiment.