PrPSc detection in the mucosal solution (A), the serosal solution (B), and the scraped mucosal layer (C) of trout everted intestine. Western blot analysis of PrPSc in the crude mice brain homogenate (positive control, +), in the diluted mice brain homogenate solution (1:20) perfonding the mucosal side of the trout everted intestine (A), in the physiological solution fluxing the serosal side of the trout everted intestine (B), and in the scraped mucosal layer of the trout intestine (C). The mucosal tissue in sample C was obtained by gently washing (1 min) the intestine with a saline cold solution then ablated and homogenized 1 hour after perfusion (C). The experiment was repeated on three different trout intestine (1, 2, 3). The classical tri-banded pattern of diglycosylated, mono- and unglycosylated PrPSc was absent in B and only barely detectable in C suggesting a low absorption of PrPSc to the mucosal side of trout intestine and the absence of an active PrPSc transport through the intestinal barrier.