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Table 5 Molecular weight estimations of PrPres form in retropharyngeal lymph nodes and brain stem*.

From: Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep

 

scrapie

 

RLN

brain stem

antibody

L42

9A2

12B2†

9A2

PrP res form

diglycosyl

28.8 ± 0.7

28.9 ± 1.9

29.2 ± 0.8

28.6 ± 1.4

monoglycosyl

22.4 ± 0.5

22.9 ± 1.0

22.8 ± 0.2

22.7 ± 0.5

aglycosyl

18.8 ± 0.4

19.0 ± 0.8

18.9 ± 0.1

18.6 ± 0.5

 

BSE

 

RLN

brain stem

antibody

L42

9A2

12B2†

9A2

PrP res form

diglycosyl

28.5 ± 1.3

28.9 ± 2.0

N.R.

28.3

monoglycosyl

21.9 ± 1.1

22.2 ± 2.1

N.R.

22.5

aglycosyl

17.8 ± 0.8

18.0 ± 1.7

N.R.

18.0

  1. * Values are in kDa and presented as average ± SD, from 3 different experiments with at least 4 different individual animals per experiment, except for mAbs 12B2 (n = 2) and 9A2 on BSE infected brain stem, n = 1.
  2. † Antibody 12B2 yielded the same MWr as P4 in scrapie samples; N.R. = no reactivity.