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Table 5 Molecular weight estimations of PrPres form in retropharyngeal lymph nodes and brain stem*.

From: Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep

  scrapie
  RLN brain stem
antibody L42 9A2 12B2 9A2
PrP res form
diglycosyl 28.8 ± 0.7 28.9 ± 1.9 29.2 ± 0.8 28.6 ± 1.4
monoglycosyl 22.4 ± 0.5 22.9 ± 1.0 22.8 ± 0.2 22.7 ± 0.5
aglycosyl 18.8 ± 0.4 19.0 ± 0.8 18.9 ± 0.1 18.6 ± 0.5
  BSE
  RLN brain stem
antibody L42 9A2 12B2 9A2
PrP res form
diglycosyl 28.5 ± 1.3 28.9 ± 2.0 N.R. 28.3
monoglycosyl 21.9 ± 1.1 22.2 ± 2.1 N.R. 22.5
aglycosyl 17.8 ± 0.8 18.0 ± 1.7 N.R. 18.0
  1. * Values are in kDa and presented as average ± SD, from 3 different experiments with at least 4 different individual animals per experiment, except for mAbs 12B2 (n = 2) and 9A2 on BSE infected brain stem, n = 1.
  2. Antibody 12B2 yielded the same MWr as P4 in scrapie samples; N.R. = no reactivity.