Skip to main content

Table 2 Sheep with preclinical scrapie*.

From: Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep

    diagnosis
sheep nr genotype age (m) IHC WB
    brain RLN brain RLN
PS-5 VRQ/VRQ (n = 18) 2 - 1+ - -
PS-6   2 - 1+ - -
PS-3   3 - 2+ - 2+
PS-4   3 - - - -
PS-1   4 - 4+ - 3+
PS-12   4 - 3+ - 2+
PS-7   5 - 4+ - 3+
PS-8   5 - 4+ - 1+
PS-10   5 - 4+ - 1+
PS-11   5 - 3+ - 2+
PS-18   10 1+§ 4+ - 2+
PS-17   13 1+ 4+ - 2+
PS-15   14 2+ 4+ - 2+
PS-16   14 2+ 4+ - 3+
PS-14   17 3+ 4+ 1+ 2+
PS-13   21 3+ 4+ 3+ 2+
PS-23   26 3+ 4+ 3+ 2+
PS-24   26 3+ 4+ 3+ 1+
PS-21 ARR/VRQ (n = 4) 17 - - - -
PS-22   17 - - - -
PS-19   24 - - - -
PS-20   24 - - - -
PS-2 ARR/ARR (n = 2) 3 - - - -
PS-9   6 - - - -
  1. * open flock of Texel cross breed with natural scrapie; – means no PrP or PrPres detected.
  2. † number of plus signs indicate % of follicles PrP-positive 1+ = <25%, 2+ = 25-50%, 3+ = 50–75%, 4+ = 75–100%.
  3. 1+ = weak signal for all three PrPres bands; 2+ = PrPres bands well visible; 3+ = PrPres bands so dark that diglycosyl and monoglycosyl are merging together at 6 min film exposure. Results obtained with either of the antibodies 12B2, P4 or 9A2 after SDS-PAGE of non-concentrated samples, or with 12B2 and concentrated samples.
  4. § In a previous study, another 10 m old RLN positive animal was found negative in the brain by IHC [25].
  5. Clinical signs.