Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep

BMC Veterinary Research

Table 1 Epitope specificities of 4 mouse monoclonal antibodies used.

antibody	elicited with sequence	epitope core by Pepscan analysis^†	blocking ELISA^‡
			89–107	145–177
12B2	bovine PrP97-115^§	93WGQGG97	yes	no
P4	ovine PrP89-104	93WGQGGSH99	yes	no
9A2	bovine PrP97-115^§	102WNK104	yes	no
L42	ovine PrP145-163	148YEDRYY153	no	yes

†Mapping of antigenic sites in the ovine PrP sequence for 4 monoclonal antibodies by Pepscan analysis.
Shown are shared sequences (core of epitope) of overlapping 15-mer antigenic peptides for antibodies 12B2, P4, 9A2 and L42 from Figure 1. Numbers refer to ovine PrP sequence Genbank accession nr. AJ567985.
^‡Blocking was 100% in all positive cases and 0% in all negative cases with peptide concentrations of ≥ 0.5 μg/ml and antibody concentrations ≤ 0.5 μg/ml.
^§Corresponds to ovine PrP sequence 89–107. Antibodies produced by [60]

ISSN: 1746-6148