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Figure 2 | BMC Veterinary Research

Figure 2

From: Evaluation of the persistence and gene expression of an anti-Chlamydophila psittaciDNA vaccine in turkey muscle

Figure 2

RT-PCR detection of mRNA in muscle tissue of vaccinated turkeys. Gel image representing the PCR results on cDNA, obtained from both vaccinated muscle tissue at the (presumed) site of injection from the day of vaccination (w0pv) to 10 weeks p.v. (lanes 3 to 13) and muscle tissue of the opposite (non-vaccinated) thigh on weeks 1, 4 and 9 p.v. (lanes 14 to 16). The SmartLadder (Eurogentec) was used as a molecular size marker (Smart). MilliQ water was used as a negative control (lanes 2 and 17) and cDNA, generated by RT-PCR from mRNA produced in vitro from pcDNA1::MOMP, as a positive control (lane 2). A band of 850 bp could be detected by gel electrophoresis from 1 to 10 weeks p.v. after PCR on cDNA from vaccinated muscle tissues, at the (presumed) site of injection (lanes 4 to 13). No PCR products were obtained after performing a PCR on cDNA from muscle tissue of the opposite (non-vaccinated) thighs (lanes 14 to 16) or muscle tissue at the site of injection at the day of vaccination (lane 3).

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