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Figure 1 | BMC Veterinary Research

Figure 1

From: Evaluation of the persistence and gene expression of an anti-Chlamydophila psittaciDNA vaccine in turkey muscle

Figure 1

PCR detection of the plasmid DNA in muscle tissue of vaccinated turkeys. Gel image representing the PCR-products, obtained by PCR on DNA extracted from muscle tissue at 8, 10 and 11 weeks p.v. (w8pv, w10pv and w11pv). The 3 different samples of the same date, visible on the gel, are 3 DNA extractions from the pooled stock of muscle tissue at the (presumed) injection site, on which the PCR was conducted. The arrows indicate where a band of 1084 bp is visible. The SmartLadder (Eurogentec) was used as molecular size marker (with bands of 200, 400, 600, 800, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000 and 10000 bp). DNA of non-vaccinated muscle tissue was used as a negative control (-) and the pcDNA1::MOMP vector as a positive control (+). At 8 weeks p.v., a band of 1084 bp could still be detected in 1 out of 3 DNA isolates and at 10 weeks p.v. in 2 out of 3 DNA isolates. At 11 weeks p.v. no band could be detected on the ethidium bromide stained gel.

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