Comparison of paraffin-embedded skin biopsies from different anatomical regions as sampling methods for detection of Leishmania infection in dogs using histological, immunohistochemical and PCR methods

Table 2 Comparison among polymerase chain reaction (PCR), immunohistochemical (IHC) and histopathological (HE) methods for Leishmania detection versus canine skin biopsies from different anatomical locations in 29 naturally infected dogs

Skin region	PCR +	(%)	IHC +	(%)	HE +	(%)
Ear	21^a	(72.4%)	14^b	(48.3%)	12^b	(41.4%)
Nose	19^a	(65.5%)	11^b	(37.9%)	5^c	(17.2%)
Abdomen	14^a	(48.3%)	11^a	(37.9%)	5^a	(17.2%)

Each data point is associated a one letter (a, b or c). Data with the same letters means no statistical difference. Otherwise, different letters means statistical difference. Each line: Letters a, b, c, represent statistical analysis considering the three methods together. Different letter were used to significant differences, chi-square test (p < 0.05).

ISSN: 1746-6148