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Table 1 Comparison of Leishmania detection by polymerase chain reaction (PCR), immunohistochemical (IHC) and histopathological (HE) methods in biopsies from distinct skin anatomical locations according to clinical status: asymptomatic (n = 10), oligosymptomatic (n = 10) and symptomatic (n = 9)

From: Comparison of paraffin-embedded skin biopsies from different anatomical regions as sampling methods for detection of Leishmaniainfection in dogs using histological, immunohistochemical and PCR methods

Method

Clinical Group

SKIN LOCATION

  

% POS (positive at last one location)

  

Ear

Nose

Abdomen

 

PCR

Asymptomatic

7

6

2

90%

 

Oligosymptomatic

7

8

5

80%

 

Symptomatic

7

5

7

78%

IHC

Asymptomatic

3

4

4

70%

 

Oligosymptomatic

4

2

2

40%

 

Symptomatic

7

5

5

78%

HE

Asymptomatic

3

2

2

30%

 

Oligosymptomatic

4

1

2

40%

 

Symptomatic

5

7

1

67%