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Table 3 Characteristics of SNPs in GUSB exons

From: Molecular characterization and exclusion of porcine GUSBas a candidate gene for congenital hernia inguinalis/scrotalis

Exon Primersa Positionb Test-Assay Resulting fragments Allele-Frequencies
5 EX5outer.f/EX5outer.r/
EX5innerG/EX5innerA
805 Tetra-primer
ARMS-PCR
G: 606, 342
A: 606, 308
G: 0.707
A: 0.293
6 Ex6Seq.f/Ex6Seq.r 1044 HpaI-PCR-RFLP T: 132, 109
C: 241
T: 0.651
C: 0.349
7 Ex7Seq.f/Ex7Seq.r 1227 HhaI-PCR-RFLP T: 295
C: 123, 118
T: 0.987
C: 0.013
  1. a Primer sequences given in table 1
  2. b Numbering refers to CDS as outlined in GenBank:DQ095863