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Table 3 Characteristics of SNPs in GUSB exons

From: Molecular characterization and exclusion of porcine GUSBas a candidate gene for congenital hernia inguinalis/scrotalis

Exon

Primersa

Positionb

Test-Assay

Resulting fragments

Allele-Frequencies

5

EX5outer.f/EX5outer.r/

EX5innerG/EX5innerA

805

Tetra-primer

ARMS-PCR

G: 606, 342

A: 606, 308

G: 0.707

A: 0.293

6

Ex6Seq.f/Ex6Seq.r

1044

HpaI-PCR-RFLP

T: 132, 109

C: 241

T: 0.651

C: 0.349

7

Ex7Seq.f/Ex7Seq.r

1227

HhaI-PCR-RFLP

T: 295

C: 123, 118

T: 0.987

C: 0.013

  1. a Primer sequences given in table 1
  2. b Numbering refers to CDS as outlined in GenBank:DQ095863
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BMC Veterinary Research

ISSN: 1746-6148

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