Figure 6

SDS-PAGE of Sepharose CL-2B peaks. Aliquots of pooled Sepharose CL-2B fractions were precipitated with 100% ethanol, dissolved in 15 μl of 100 mM Tris buffer, adjusted to pH 8.0 with concentrated acetic acid and digested with 20 mU of chondroitinase ABC at 37°C for 24 h. Chondroitinase ABC digested samples and non-digested replicate aliquots were separated on silver stained 10% SDS-polyacrylamide gels. Lane 1: untreated sample from control SDFT no. 1; lane 2: untreated sample from DSLD SDFT no.8 (Table 1); lane 3: chondroitinase ABC treated sample from control SDFT no. 1; lane 4: chondroitinase ABC treated sample from SDFT no. 8 (Table 1).