Table 2 Primer sequences and predicted size of amplification products
Target gene coding for virulence factors | Oligonucleotide sequences of primers | Amplified Products (bp) | Primer Coordinates | Accession number | Reference |
|---|---|---|---|---|---|
LT | 5'-ATT TAC GGC GTT ACT ATC CTC-3' 5'-TTT TGG TCT CGG TCA GAT ATG-3' | 281 | 27–47 287–307 | S60731 | Osek et al. [35] |
STaa | 5'-TCC GTG AAA CAA CAT GAC GG-3' 5'-ATA ACA TCC AGC ACA GGC AG-3' | 244 | 267–286j 492–510 | M58746 | Ojeniyi et al. [34] |
STb | 5'-GCC TAT GCA TCT ACA CAA TC-3' 5'-TGA GAA ATG GAC AAT GTC CG-3' | 279 | 515–534 773–793 | AY028790 | Ojeniyi et al. [34] |
Stx1allb | 5'-CGC TGA ATG TCA TTC GCT CTG C-3' 5'-CGT GGT ATA GCT ACT GTC ACC-3' | 302 | 113–134 394–414 | M17358 | Blanco et al. [32] |
Stx2allc | 5'-CTT CGG TAT CCT ATT CCC GG-3' 5'-CTG CTG TGA CAG TGA CAA AAC GC-3' | 516 | 50–69 543–565 | M59432 | Blanco et al. [32] |
Stx2e | 5-ATG AAG AAG ATG TTT ATA GCG-3' 5'-TCA GTT AAA CTT CAC CTG GGC-3' | 264 | 1176–1196 1419–1439 | M36727 | Osek et al. [35] |
EAST1 | 5'-CCA TCA ACA CAG TAT ATC CGA-3' 5'-GGT CGC GAG TGA CGG CTT TGT-3' | 111 | 2–24 94–114 | S81691 | Yamamoto and Nakazawa [18] |
F4 (K88)d | 5'-GCT GCA TCT GCT GCA TCT GGT ATG G-3' 5'-CCA CTG AGT GCT GGT AGT TAC AGC C-3' | 792 | 31–54 798–822 | M29374 | This study |
F5 (K99) | 5'-TGC GAC TAC CAA TGC TTC TG-3' 5'-TAT CCA CCA TTA GAC GGA GC-3' | 450 | 45–64 475–494 | M35282 | Ojeniyi et al. [34] |
F6 (P987) | 5'-TCT GCT CTT AAA GCT ACT GG-3' 5'-AAC TCC ACC GTT TGT ATC AG-3' | 333 | 193–212 506–525 | M35257 | Ojeniyi et al. [34] |
F17e | 5'-GGG CTG ACA GAG GAG GTG GGG C-3' 5'-CCC GGC GAC AAC TTC ATC ACC GG-3' | 411 | 289–310 677–699 | AF055313 | This study |
F18f | 5'-GTG AAA AGA CTA GTG TTT ATT TC-3' 5'-CTT GTA AGT AAC CGC GTA AGC-3' | 510 | 1–23 490–510 | M61713 | Imberechts et al. [33] |
F41 | 5'-GAG GGA CTT TCA TCT TTT AG-3' 5'-AGT CCA TTC CAT TTA TAG GC-3' | 431 | 154–173 565–584 | X14354 | Ojeniyi et al. [34] |
eae g | 5'-GGA ACG GCA GAG GTT AAT CTG CAG-3' 5'-GGC GCT CAT CAT AGT CTT TC-3' | 775 | 1441–1460 2193–2215 | AF022236 | Blanco et al. [32] |