Quantification of representative miRNAs of Ascaris lumbricoides and A . suum . Three novel miRNAs of each nematode were detected. Two miRNAs named asu-miR-novel-031 and alu-miR-novel-53 had significant expression differences of 5p and 3p mature miRNAs. The alu-miR-novel-64-5p and asu-miR-novel-383-3p were not successfully amplified, which might due to their low expression levels.