Transcriptional approach to study porcine tracheal epithelial cells individually or dually infected with swine influenza virus and Streptococcus suis

Table 1 Primer sequences used for real-time RT-qPCR

Gene	Genebank ID	Amplicon size	Forward sequence (primer #)	Reverse sequence (primer #)	Efficiency (qPCR)
*Hipox*	NM_001032376	142 bp	GCAGCCCCAGCGTCGTGATT	CGAGCAAGCCGTTCAGTCCTGT	99
*Ppia*	NM_214353	133 bp	TGCAGACAAAGTTCCAAAGACAG	GCCACCAGTGCCATTATGG	97
*Ccl5*	NM_001129946	78 bp	GAAATGGGTGCGGGAGTACA	GTTTGCACGAGTTCAGGCTC	94
*Cox2*	NM_214321	165 bp	TAGGATTCAGGGCTTTCACTGGCT	TGTCAGCCGACAATGAGATGTGGA	105
*Ifn*β	NM_001003923	150 bp	TGCAACCACCACAATTCCAGAAGG	TCTGCCCATCAAGTTCCACAAGGA	102
*Il1a*	NM_214029	152 bp	TGAAGATGGCCAAAGTCCCTGACCT	ATCCATGCCGTCCCCAGGAAGTG	93
*Il6*	NM_214399	105 bp	ACTCCCTCTCCACAAGCGCCTT	TGGCATCTTCTTCCAGGCGTCCC	97
*Il8*	NM_213867	80 bp	TGTGAGGCTGCAGTTCTGGCAAG	GGGTGGAAAGGTGTGGAATGCGT	95
*Il12a*	NM_213993	162 bp	CTGAAGGCCGTCAGCAACAC	AGCCAGGCAACTCTCATTCG	92
*Ifn*λ1	NM_001142837	135 bp	TGGCCTTAGAGGCTGAGCTA	CCCTGATGCAAGCCTGAAGT	91
*Irf1*	NM_001097413	113 bp	AATCCAGCCCTGATACCTTCTCT	GGCCTGTTCAATGTCCAAGTC	95
*Irf7*	NM_001097428	94 bp	CTGCGATGGCTGGATGAA	TAAAGATGCGCGAGTCGGA	90
*Plau*	NM_213945	143 bp	CTCCAAAGGCAGCCATGAAC	CACAGTGCTCCCCTTGGAAT	106
*Tnf*	NM_214022	112 bp	GCCACCACGCTCTTCTGCCTA	ACGATGATCTGAGTCCTTGGGCCA	91
*Vcam1*	NM_213891	126 bp	TCCACGCTGGTCATGAATCC	TGGGTCCTTGGGGAAAGAGTA	101

ISSN: 1746-6148