Antibody response to BoHV-1 observed in vaccinated cattle. BoHV-1-specific antibody response detected by ELISA (b) or virus neutralization assay (a) in animals vaccinated either with inactivated (I) or live attenuated vaccine (L) formulated with BoHV-1ΔgEβgal or BoHV-1 LA viruses. Live attenuated vaccines were administered by intranasal (in), intramuscular (im) or intravenous (iv) route. Neutralizing antibody titers are expressed as log10 of the reciprocal value of the serum dilution giving protection to CPE. Averages and SEM of the titers obtained for each group are shown for each time point. Antibody titers measured by ELISA are expressed as log10 of the reciprocal value of the serum dilution giving an A405 of 40% of the positive control. Each point represents the mean titer and SEM of five animals.